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Articles from Springer a leading global scientific publisher of scientific books and journals. - dna forensic @ Tue, 22 Aug 2017 at 04:59 PM
Forensic Science - Encyclopedia of Law and Economics @ 2021-01-01
Forensic science applies natural, physical, and social sciences to resolve legal matters. The term forensics has been attached to many different fields: economics, anthropology, dentistry, pathology, toxicology, entomology, psychology, accounting, engineering, and computer forensics. Forensic evidence is gathered, examined, evaluated, interpreted, and presented to make sense of an event and provide investigatory leads. Various classification schemes exist for forensic evidence, with some forms of evidence falling under more than one scheme. Rules of evidence differ between jurisdictions, even between countries that share similar legal traditions. This makes the sharing of evidence between countries particularly problematic, at times rendering this evidence inadmissible in national courts. Several measures have been proposed and organizations created to strengthen forensic science and promote best practices for practitioners, researchers, and academicians in the field.
 
Genetics and Tropical Forests - Tropical Forestry Handbook @ 2021-01-01
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Genetics and Tropical Forests - Tropical Forestry Handbook @ 2021-01-01
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Fluorescence Lifetime Imaging - Handbook of Photonics for Biomedical Engineering @ 2021-01-01
Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environment and interaction of fluorescent probes. It can report on photophysical events that are difficult or impossible to observe by fluorescence intensity imaging, because FLIM is largely independent of the local fluorophore concentration and excitation intensity. Many FLIM applications relevant for biology concern the identification of Förster resonance energy transfer (FRET) to study protein interactions and conformational changes. In addition, FLIM has been used to image viscosity, temperature, pH, refractive index, and ion and oxygen concentrations, all at the cellular level. The basic principles and recent advances in the application of FLIM, FLIM instrumentation, molecular probe, and FLIM detector development will be discussed.
 
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Introduction - Development of a Fully Integrated “Sample-In-Answer-Out” System for Automatic Genetic Analysis @ 2018-01-01
The tradition classic approach toward genetic approach is present. Several methods for DNA extraction, amplification and electrophoresis are enumerated. Different kinds of approaches have their own advantages and application occasions. Microfluidic is a newly developed technology that aims at precisely manipulating fluidic of micro-scale. The microfluidic made it possible to integrate tradition genetic analysis process onto one single microchip for automation. Several trials have been made to transplant tradition process onto microfluidic platform, although various weak points restricted them from further industrialization. This chapter will give a brief introduction of the standard three-step process of genetic analysis on each step followed by a detailed expatiation on the development of microfluidic technology. All steps of genetic analysis, extraction, amplification and detection, have all been transplanted on-chip separately, and further researches also reported the integration of partial process. Several integrated systems are also introduced, along with careful evaluations. At the end of chapter, the significance of this study will be stated, and mainlines of following chapters will be addressed.
 
Construction of a General Platform for Capillary Electrophoresis - Development of a Fully Integrated “Sample-In-Answer-Out” System for Automatic Genetic Analysis @ 2018-01-01
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Construction of a General Platform for Capillary Electrophoresis - Development of a Fully Integrated “Sample-In-Answer-Out” System for Automatic Genetic Analysis @ 2018-01-01
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Integrated Module for Automated DNA Extraction and Amplification - Development of a Fully Integrated “Sample-In-Answer-Out” System for Automatic Genetic Analysis @ 2018-01-01
An integrated module for automated sample preparation is present in this chapter. A disposable PMMA chip, together with related instrument to manipulate the chip, was designed and fabricated. On-chip extraction was performed by twining long-chain nucleic acid molecules onto the filter paper. And the thermal cycles were then applied directly to the filter paper with DNA twined on for PCR amplification. To precisely manipulate on-chip fluidic and fully seal PCR chamber, an on-chip valve structure was designed and verified. Unlike classic pneumatic valve for glass chip, this valve requires no off-chip pneumatic components and can be simply driven by a pushing rod. And with an adjusted thermal bonding protocol, the valve structure can be simply fabricated. A LabView software was also programmed to control the fluidic and temperature of the system. With the chip, instrument and program, on-chip integrated and automated sample preparation was performed and the limit of detection was determined. The system was then applied for fast screening of congenital hearing loss gene and the results turned out satisfactorily.
 
Conclusions and Prospects for Future Work - Development of a Fully Integrated “Sample-In-Answer-Out” System for Automatic Genetic Analysis @ 2018-01-01
All through this book, we hope we do offer readers some enlightenments or inspirations for their works. We will conclude our research works in this chapter and offer some prospects for future works. We hope the system would be able to integrate more modules, deal with more various kinds of samples and be applied in more areas. We would be quite happy if our research works do help more and more people.
 
Found 11 Articles for dna forensic